| Prof.
Yong Kun Park, M.D. Ph.D. Médico patologista, bioquímico professor da Faculdad de Ingeniería de alimentos de UNICAMP. Investigador visitante en diversos centros de investigación del mundo como; Rochester University, North Carolina University, Centro de control de enfermedades de las fuerzas armadas americanas, etc. Su principal interes es el uso de propoleos en biotecnologia e farmacologia. |
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| Items 1 - 14 of 14 |
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| 1: J Agric Food Chem. 2005 Dec 28;53(26):10306-9. |
Suppressive effects of ethanolic
extracts from propolis and its main botanical origin
on dioxin toxicity.
Park
YK, Fukuda
I, Ashida
H, Nishiumi
S, Yoshida
K, Daugsch
A, Sato
HH, Pastore
GM.
Department of Food Science,
Suppressive effects of ethanolic extracts prepared
from propolis group 12 and its main botanical origin
(leaf bud of Baccharis dracunculifolia)
on transformation of the aryl hydrocarbon receptor (AhR),
the initial action of dioxin toxicity, were investigated. It was found that
suppressive effects of propolis on AhR transformation were relatively higher than those of resins
of its botanical origin in cell-free system and in Hepa-1c1c7 cells. When
the composition of chemical ingredients was measured, propolis
contained slightly higher amounts of flavonoid aglycones as compared with its botanical origin with the same
characteristics. Moreover, antiradical activity, one of the typical biological
activities of flavonoids, in propolis was also slightly higher than that in its botanical
origin. These results indicate that not only propolis
but also its botanical origin contains high amounts of flavonoid
aglycones and that both of them are useful dietary
sources for flavonoids with a potency to prevent
dioxin toxicity.
PMID: 16366731 [PubMed
- indexed for MEDLINE]
| 2: J Agric Food Chem. 2005 Feb 23;53(4):1166-72. |
Some chemical composition and biological activity
of northern Argentine propolis.
Isla MI, Paredes-Guzman JF, Nieva-Moreno MI,
Koo
H, Park
YK.
Department
of Food Science,
Twenty-five samples of propolis were collected from
seven different regions in northern
PMID: 15713035 [PubMed
- indexed for MEDLINE]
| 3: Biosci Biotechnol Biochem. 2004 Apr;68(4):935-8. |
Suppression
of dioxin mediated aryl hydrocarbon receptor transformation by ethanolic
extracts of propolis.
Park
YK, Fukuda
I, Ashida
H, Nishiumi
S, Guzman
JP, Sato
HH, Pastore
GM.
Present study demonstrated that the ethanolic extracts
of propolis containing higher concentrations of
flavonoids suppressed 2,3,7,8-tetrachlorodibenzo-p-dioxin
(TCDD)-induced aryl hydrocarbon receptor transformation in a dose-dependent
manner. The IC(50) values of propolis group 3 and
group 12 were 1.2 and 3.6 microg/ml, respectively,
indicating that propolis showed stronger antagonistic
effects as compared with vegetable extracts.
| 4: J Agric Food Chem. 2004 Mar 10;52(5):1100-3. |
Chemical constituents in Baccharis dracunculifolia as the
main botanical origin of southeastern Brazilian
propolis.
Park YK, Paredes-Guzman JF,
Aguiar CL, Alencar SM, Fujiwara FY.
Department
of Food Science,
Previously, it was reported that one group of propolis
(Group 12) was identified in southeastern
PMID: 14995105 [PubMed
- indexed for MEDLINE]
| 5: Oral Microbiol Immunol. 2002 Dec;17(6):337-43. |
Effects of apigenin
and tt-farnesol on glucosyltransferase
activity, biofilm viability and caries development
in rats.
Koo H, Pearson
SK, Scott-Anne
K, Abranches J, Cury JA, Rosalen PL, Park
YK, Marquis
RE, Bowen
WH.
Center
for Oral Biology and Eastman Department of Dentistry, University of
Propolis, a resinous hive product secreted by Apis mellifera bees, has been shown
to reduce the incidence of dental caries in rats. Several compounds, mainly
polyphenolics, have been identified in propolis. Apigenin and tt-farnesol demonstrated biological activity against mutans streptococci. We determined here their effects, alone
or in combination, on glucosyltransferase activity,
biofilm viability, and development of caries in
rats. Sprague-Dawley rats were infected with Streptococcus
sobrinus 6715 and treated topically twice daily
as follows: (1) tt-farnesol, (2) apigenin, (3) vehicle control, (4) fluoride, (5) apigenin +tt-farnesol, and (6) chlorhexidine. Apigenin (
| 6: Caries Res. 2002 Nov-Dec;36(6):445-8. |
Effect of a mouthrinse
containing selected propolis on 3-day dental plaque
accumulation and polysaccharide formation.
Koo H, Cury JA, Rosalen PL, Ambrosano GM, Ikegaki M, Park
YK.
Department of Dentistry, University of
The aim of this study was to evaluate the effect of a mouthrinse
containing propolis SNB-RS on 3-day dental plaque
accumulation. Six volunteers took part in a double-blind crossover study performed
in two phases of 3 days. During each phase the volunteers refrained from all
oral hygiene and rinsed with 20% sucrose solution 5 times a day to enhance
dental plaque formation and with mouthrinse (placebo
or experimental) twice a day. On the 4th day, the plaque index (PI) of the
volunteers was scored and the supragingival dental
plaque was analyzed for insoluble polysaccharide (IP). The PI (SD) for the
experimental group was 0.78 (0.17), significantly less than for the placebo
group, 1.41 (0.14). The experimental mouthrinse
reduced the IP concentration in dental plaque by 61.7% compared to placebo
(p < 0.05). An experimental mouthrinse containing
propolis SNB-RS was thus efficient in reducing supragingival plaque formation and IP formation under conditions
of high plaque accumulation. Copyright 2002
S. Karger AG, Basel
Publication Types:
PMID: 12459618 [PubMed - indexed for MEDLINE]
| 7: Antimicrob Agents Chemother. 2002 May;46(5):1302-9. |
Effects
of compounds found in propolis on Streptococcus
mutans growth and on glucosyltransferase activity.
Koo
H, Rosalen
PL, Cury
JA, Park
YK, Bowen
WH.
Center for Oral Biology and Eastman Department of
Dentistry, University of Rochester Medical Center,
Rochester, New York 14642, USA. Hyun_Koo@urmc.rochester.edu
Propolis, a resinous bee product, has been shown
to inhibit the growth of oral microorganisms and
the activity of bacterium-derived glucosyltransferases
(GTFs). Several compounds, mainly polyphenolics,
have been identified in this natural product. The present study evaluated
the effects of distinct chemical groups found in propolis
on the activity of GTF enzymes in solution and on the surface of saliva-coated
hydroxyapatite (sHA) beads. Thirty
compounds, including flavonoids, cinnamic
acid derivatives, and terpenoids, were tested for
the ability to inhibit GTFs B, C, and D from Streptococcus
mutans and GTF from S. sanguinis (GTF Ss). Flavones and flavonols
were potent inhibitors of GTF activity in solution; lesser effects were noted
on insolubilized enzymes. Apigenin,
a 4',5,7-trihydroxyflavone, was the most effective
inhibitor of GTFs, both in solution (90.5 to 95%
inhibition at a concentration of 135 microg/ml)
and on the surface of sHA beads (30 to 60% at 135
microg/ml). Antibacterial activity was determined by using
MICs, minimum bactericidal concentrations (MBCs), and time-kill studies. Flavanones
and some dihydroflavonols, as well as the sesquiterpene tt-farnesol, inhibited
the growth of S. mutans and S. sobrinus; tt-farnesol was the most
effective antibacterial compound (MICs of 14 to
28 microg/ml and MBCs
of 56 to 112 microg/ml). tt-Farnesol (56 to 112 microg/ml) produced a 3-log-fold reduction in the bacterial
population after 4 h of incubation. Cinnamic acid
derivatives had negligible biological activities. Several of the compounds
identified in propolis inhibit GTF activities and
bacterial growth. Apigenin is a novel and potent
inhibitor of GTF activity, and tt-farnesol was found
to be an effective antibacterial agent.
PMID: 11959560 [PubMed
- indexed for MEDLINE]
| 8: J Agric Food Chem. 2002 Apr 24;50(9):2502-6. |
Botanical origin and chemical composition
of Brazilian propolis.
Park
YK, Alencar
SM, Aguiar
CL.
Department of Food Science,
Brazilian propolis has been classified into 12 groups
based on physicochemical characteristics: five in the southern
PMID: 11958612 [PubMed
- indexed for MEDLINE]
| 9: J Nat Prod. 2001 Oct;64(10):1278-81. |
Anti-AIDS agents.
48.(1) Anti-HIV activity of moronic acid derivatives and the
new melliferone-related triterpenoid
isolated from Brazilian propolis.
Ito
J, Chang
FR, Wang
HK, Park
YK, Ikegaki M, Kilgore
N, Lee
KH.
Natural Products Laboratory,
A new triterpenoid named melliferone
(1), three known triterpenoids, moronic acid (2),
anwuweizonic acid (3), and betulonic
acid (4), and four known aromatic compounds (5-8) were isolated from Brazilian
propolis and tested for anti-HIV activity in H9 lymphocytes.
Moronic acid (2) showed significant anti-HIV activity (EC(50)
<0.1 microg/mL, TI >186) and was modified
to develop more potent anti-AIDS agents.
PMID: 11678650 [PubMed
- indexed for MEDLINE]
| 10: Caries Res. 2000 Sep-Oct;34(5):418-26. |
Effects
of Apis mellifera propolis
on the activities of streptococcal glucosyltransferases in solution and adsorbed onto saliva-coated
hydroxyapatite.
Koo
H, Vacca
Smith AM, Bowen
WH, Rosalen
PL, Cury
JA, Park
YK.
Faculty of Dentistry of
Propolis, a resinous hive product collected by Apis mellifera bees, has been used
for thousands of years in folk medicine. Ethanolic
extracts of propolis (EEP) have been shown to inhibit
the activity of a mixture of crude glucosyltransferase
(Gtf) enzymes in solution. These enzymes synthesize
glucans from sucrose, which are important for the formation
of pathogenic dental plaque. In the present study, the effects of propolis from two different regions of
| 11: Curr Microbiol. 2000 Sep;41(3):192-6. |
Effect of a new variety of Apis mellifera propolis on mutans Streptococci.
Koo H, Rosalen PL, Cury JA, Ambrosano GM,
Murata RM, Yatsuda R, Ikegaki M, Alencar SM, Park YK.
Department of Physiological Sciences,
Faculty of Dentistry of Piracicaba, State University
of Campinas, Caixa Postal
52, Piracicaba, 13414-900, SP, Brazil. Hyun-Koo@urmc.rochester.edu
The effects of a new variety of propolis, from Northeastern Brazil (BA), on growth of mutans
streptococci, cell adherence, and water-insoluble glucan
(WIG) synthesis were evaluated. Propolis from Southeastern (MG) and Southern (RS)
PMID: 10915206 [PubMed
- indexed for MEDLINE]
| 12: Arch Oral Biol. 2000 Feb;45(2):141-8. |
In
vitro antimicrobial activity of propolis and Arnica
Koo H, Gomes
BP, Rosalen PL, Ambrosano GM, Park
YK, Cury JA.
Arnica and propolis have been used for thousands
of years in folk medicine for several purposes. They possess several biological
activities such as anti-inflammatory, antifungal, antiviral and tissue regenerative,
among others. Although the antibacterial activity of propolis has already been demonstrated, very few studies have
been done on bacteria of clinical relevance in dentistry. Also, the antimicrobial
activity of Arnica has not been extensively investigated. Therefore the aim
here was to evaluate in vitro the antimicrobial activity, inhibition of adherence
of mutans streptococci and inhibition of formation
of water-insoluble glucan by Arnica and propolis extracts. Arnica
| 13: Caries Res. 1999 Sep-Oct;33(5):393-400. |
Effect of Apis mellifera propolis from two Brazilian
regions on caries development in desalivated rats.
Koo H, Rosalen PL, Cury JA, Park
YK, Ikegaki M, Sattler
A.
Department of Physiological Sciences, Faculty of Dentistry
of
The purpose of the present study was to evaluate the effect of Apis mellifera propolis collected from two regions of
| 14: Curr Microbiol. 1998 Jan;36(1):24-8. |
Antimicrobial activity of propolis on oral microorganisms.
Park
YK, Koo MH, Abreu JA, Ikegaki M, Cury JA, Rosalen PL.
Formation of dental caries is caused by the colonization and accumulation
of oral microorganisms and extracellular
polysaccharides that are synthesized from sucrose by glucosyltransferase
of Streptococcus mutans. The production of glucosyltransferase from oral microorganisms
was attempted, and it was found that Streptococcus mutans
produced highest activity of the enzyme. Ethanolic
extracts of propolis (EEP) were examined whether
EEP inhibit the enzyme activity and growth of the bacteria or not. All EEP
from various regions in